Primer3Plus
pick primers from a DNA sequence
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Mark selected region:
 
Excluded Regions: <  >
Targets: [  ]
Included Region: {  }
Primer overlap positions: -
Pair OK Region List:

Pick left primer Pick hybridization probe
Pick right primer
or use left primer below. (internal oligo) or use oligo below. or use right primer
below (5'->3' on opposite strand).
        
  5' Overhang:      5' Overhang: